High-throughput three-dimensional lithographic microfabrication

High-throughput three-dimensional (3D) lithographic microfabrication in biomedical applications

Two-photon excitation microfabrication has been shown to be useful in the field of photonics and biomedicine. It generates 3D microstructures and provides sub-diffraction fabrication resolution. Nevertheless, laser direct writing, the most popular two-photon fabrication technique, has slow fabrication speed, and its applications are limited to prototyping. In this proceeding, we propose high-th...

Assembly and Molding Processes for Three-dimensional Microfabrication Assembly and Molding Processes for Three-dimensional Microfabrication Assembly and Molding Processes for Three-dimensional Microfabrication

Lithographic semiconductor microfabrication has been employed with great success for the manufacture of mechanical structures, achieving microscopic dimensions and great complexity at low cost. However, due to the inherently planar nature of the lithographic process, this technology is limited in its capability to produce three-dimensional structures. In this work, three strategies are presente...

Three-Dimensional Microfabrication by Two-Photon Polymerization

Three-dimensional cellular microarray for high-throughput toxicology assays.

We have developed a miniaturized 3D cell-culture array (the Data Analysis Toxicology Assay Chip or DataChip) for high-throughput toxicity screening of drug candidates and their cytochrome P450-generated metabolites. The DataChip consists of human cells encapsulated in collagen or alginate gels (as small as 20 nl) arrayed on a functionalized glass slide for spatially addressable screening agains...

Three-dimensional microtissue assay for high-throughput cytotoxicity of nanoparticles.

Traditional in vitro nanotoxicity researches are conducted on cultured two-dimensional (2D) monolayer cells and thereby cannot reflect organism response to nanoparticle toxicities at tissue levels. This paper describes a new, high-throughput approach to test in vitro nanotoxicity in three-dimensional (3D) microtissue array, where microtissues are formed by seeding cells in nonsticky microwells,...